[HTML][HTML] The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of …

B Li, S Liang, M Alariqi, F Wang, G Wang… - Plant biotechnology …, 2021 - ncbi.nlm.nih.gov
B Li, S Liang, M Alariqi, F Wang, G Wang, Q Wang, Z Xu, L Yu, MN Zafar, L Sun, H Si…
Plant biotechnology journal, 2021ncbi.nlm.nih.gov
… However, the temperature sensitivity of LbCpf1 has not been tested in cotton yet, a high
temperature‐resistant crop. In order to improve LbCpf1 efficiency and determine the
optimum temperature for cotton genome editing, we investigated the effects of different
temperatures on LbCpf1 activity and genome editing efficiency. Moreover, we created
nontransgenic and glandless cotton plants with seeds free of gossypol, representing a
valuable germplasm resource for cotton breeding. … The application of CRISPR/ LbCpf1mediated …
accepted 11 August 2020.* Correspondence (Tel+ 8613871599677; fax 862787280019; emails jsx@ mail. hzau. edu. cn and xlzhang@ mail. hzau. edu. cn).† These two authors contribute equally to this project.
ncbi.nlm.nih.gov
以上显示的是最相近的搜索结果。 查看全部搜索结果